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ABSTRACT
Secondary lymphoedema is one of the common complications after lymph node dissection for gynecologic malignancies and breast cancer. In this study, the relationship between PLA2 and postoperative lymphoedema in cancer at the molecular level has been explored through transcriptomics and metabolomic assays. Transcriptome sequencing technology, as well as metabolomic assays, were utilized to explore the expression of PLA2 in lymphoedema patients, and search for potential pathways in the pathogenesis and exacerbation mechanism of lymphoedema. The effect of sPLA2 on human lymphatic endothelial cells was investigated by culturing human lymphatic endothelial cells. Secretory phospholipases A2 (sPLA2) showed high expression levels in lymphoedema tissues, however, cytoplasmic phospholipases A2 (cPLA2), showed low expression in lymphoedema, as demonstrated by RT-qPCR. By culturing human lymphatic vascular endothelial cells, the study found that sPLA2 causes HLEC vacuolization and has an inhibitory effect on HLEC proliferation and migration. By detecting sPLA2 in the serum of lymphoedema patients and analyzing clinical data, it was found that sPLA2 was positively correlated with the severity of lymphoedema. Secretory Phospholipase A2 (sPLA2) is highly expressed in lymphoedema tissue, damages lymphatic vessel endothelial cells, is strongly associated with disease severity, and can be used as a potential predictor of disease severity.
Abbreviations: PLA2: Phospholipase A2; DEGs: differentially expressed genes; DMP: differential metabolic production
Acknowledgements
We would like to thank all the staff in the Department of Lymphatic Surgery, Beijing Shijitan Hospital of Capital Medical University for their contribution to our research.
We would like to thank all the staff in the Department of Joint Surgery, Beijing Shijitan Hospital of Capital Medical University for their contribution to our research.
We would like to thank all the staff at the Center for Obesity and Metabolic Disease, Beijing Shijitan Hospital of Capital Medical University for their contribution to our research.
Disclosure statement
No potential conflict of interest was reported by the author(s).
Author contributions
Conceptualization: Wenbin Shen, Nengwei Zhang
Data curation: Zimin Zhao, Wanchun Su, Jianfeng Xin, Xin Liu
Formal analysis: Zimin Zhao, Wanchun Su, Hanchen Li, Song Xia, YuGung Sun
Methodology: Zimin Zhao,
Supervision: Wenbin Shen, Nengwei Zhang, Qi Yao
Writing – original draft: Zimin Zhao
Writing – review & editing: Zimin Zhao,
Availability of data and materials
The datasets generated during and/or analyzed during the current study are available from the corresponding author upon reasonable request.
Ethics approval and consent to participate
This study was approved by the Ethics Committee and Institutional Review Board of Beijing Shijitan Hospital, Capital Medical University, in compliance with the Declaration of Helsinki (No. sjtky11-1x-2022(076)).
Additional information
Funding
Notes on contributors
Zimin Zhao
Zimin Zhao, PhD, Department of Lymphatic Surgery, Capital Medical University Affiliated Beijing Shijitan Hospital, Beijing, China, [email protected]
Wanchun Su
Wanchun Su, PhD, Department of Lymphatic Surgery, Capital Medical University Affiliated Beijing Shijitan Hospital, Beijing, China, [email protected]
Hanchen li
Hanchen li, Master's Degree, Department of Joint Surgery, Capital Medical University Affiliated Beijing Shijitan Hospital, Beijing, China, [email protected]
Xin Liu
Xin Liu, PhD, Department of Lymphatic Surgery, Capital Medical University Affiliated Beijing Shijitan Hospital, Beijing, China, [email protected]
Jianfeng Xin
Jianfeng Xin, Master's Degree, Department of Lymphatic Surgery, Capital Medical University Affiliated Beijing Shijitan Hospital, Beijing, China, [email protected]
Song Xia
Song Xia, Bachelor degree, Department of Lymphatic Surgery, Capital Medical University Affiliated Beijing Shijitan Hospital, Beijing, China, [email protected]
Yuguang Sun
Yuguang Sun, Master's Degree, Department of Lymphatic Surgery, Capital Medical University Affiliated Beijing Shijitan Hospital, Beijing, China, [email protected]
Qi yao
Qi Yao, PhD, Department of Joint Surgery, Capital Medical University Affiliated Beijing Shijitan Hospital, Beijing, China,[email protected]
Wenbin Shen
Wenbin Shen, Bachelor degree, Department of Lymphatic Surgery, Capital Medical University Affiliated Beijing Shijitan Hospital, Beijing, China, [email protected]
Nengwei Zhang
Nengwei Zhang, Master's Degree, General Surgery Department, Capital Medical University Affiliated Beijing Shijitan Hospital, Beijing, China, [email protected]