In vitro/ in vivo evaluation of double crosslinked bone glue with different degrees

ABSTRACT

Successful bone fragment fixation is a crucial factor in bone fracture healing, the fixation of crushed bone fragments could hinder bone fracture healing. Thus, ideal bone glues to effectively adhere and splice comminuted bone fragments are needed in clinical. Herein, an osteoinductive and biodegradable double cross-linked bone glue (GelMA-oDex-AMBGN) was constructed through Schiff’s base reaction between commercial GelMA (with different substitution degrees of amino groups) and Odex mixed with amine-modified mesoporous bioactive glass nanoparticles (AMBGN), followed by crosslink with blue light irradiation. The GelMA-oDex-AMBGN bone glue successfully adhered and spliced the comminuted bone fragments of isolated rat skulls. GelMA-oDex-AMBGN promoted the proliferation of 3T3 cells and enhanced the expression of osteogenic proteins Runx2 and OCN in vitro. In rat cranial critical-sized defect models, GelMA-oDex-AMBGNs with different substitution degrees significantly increased the new bone contents at the fracture defect sites and promoted bone tissue regeneration in vivo. In conclusion, the double cross-linked bone glue (GelMA-oDex-AMBGN) was successfully constructed and can induce bone regeneration. Additionally, there was no significant difference in osteogenic activity among GelMA-oDex-AMBGNs with different substitution degrees and the equal content of AMBGN.

KEYWORDS:

• Substitution degree
• osteogenesis
• double cross-linked
• GelMA

Disclosure statement

No potential conflict of interest was reported by the authors.

Data sharing agreement

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Additional information

Funding

The article was supported by the Shanxi Provincial Health Department of Science and Technology Research Projects（2022051 and 2021XM10）and Shanxi Provincial Department of Science and Technology Project (202203021211030)

Notes on contributors

Wei Fan

Wei Fan, works in the Department of Orthopedics at the Second Hospital of Shanxi Medical University and is currently pursuing a doctoral degree at Suzhou University. Proficient in the treatment of spinal degenerative diseases in orthopedics.

Yijie Liu

Yijie Liu, works at the First Affiliated Hospital of Suzhou University and is currently pursuing a doctoral degree at Suzhou University. Proficient in the treatment of spinal degenerative diseases in orthopedics.

Fushan Hou

Fushan Hou, Ph.D. in Orthopedics, works in the Orthopedics Department of the Second Hospital of Shanxi Medical University. Proficient in diagnosing and treating ankle trauma diseases.

Feng Zhao

Feng Zhao, works at the Second Hospital of Shanxi Medical University. Proficient in the diagnosis and treatment of common spinal diseases in orthopedics.

Binqiang Wu

Binqiang Wu, holds a Master's degree in Medicine and works at the Second Hospital of Shanxi Medical University. Proficient in the diagnosis and treatment of common spinal diseases in orthopedics.

Weimin Jiang

Weimin Jiang, working at the First Affiliated Hospital of Suzhou University, is the chief physician and doctoral supervisor. Proficient in the diagnosis and treatment of difficult spinal diseases in orthopedics, Director of Orthopedics, Dushu Lake Hospital, Suzhou University.