Abstract
4-Hydroxy-2-alkenals are cytotoxic aldehydes generated from the oxidation of n-3 and n-6 polyunsaturated fatty acids. The compounds have shown various biological effects via Schiff base adducts or Michael addition adducts at levels higher than physiological ones. To assess human exposure to 4-hydroxy-2-alkenals in the diet, 4-hydroxy-2-alkenals in vegetable oils, fish and shellfish were monitored using GC/MS/SIM. 2001 National Health and Nutrition Survey (2002) data were employed for the dietary intake pattern. The Korean daily exposure to 4-hydroxy-2-alkenals, excluding a possible one from fried food, was 4.3 µg day−1, constituted of 1.6 µg 4-hydroxy-2-hexenal (HHE) and 2.7 µg 4-hydroxy-2-nonenal (HNE). It was calculated that Koreans could be additionally exposed to more than 11.8 µg day−1 4-hydroxy-2-alkenal from fried foods. Thus, the combined exposure would be 16.1 µg day−1, which corresponds to 0.3 µg kg−1 body weight day−1 for a 60 kg Korean adult. In spite of the biological toxicity of 4-hydroxy-2-alkenals, the risk for human could not be quantified due to the lack of a virtually safe dose of the compounds. However, considering the basal level of 4-hydroxy-2-alkenals in many tissues, the present value from the diet may not pose a significant risk for human health.
Acknowledgements
Research was (partly) supported by a grant from the Research Institute of Human Ecology, Seoul National University.
Notes
Present address: Department of Food Science, University of Massachusetts, Amherst, MA, USA.