A comparison of the physicochemical and immunological properties of the plant gum exudates of Acacia Senegal (gum arabic) and Acacia seyal (gum tahla)∗

Dedicated to the memory of Gabriel B. J. Chapola.

Abstract

The physicochemical and immunological properties of three Sudanese gum arabic samples and four gum tahla samples (two Sudanese, one West African and one Tanzanian—Acacia seyal var. seyal) were compared. The optical rotation (ca ‐30°) and rhamnose (12–14%), arabinose (24–29%), galactose (36–42%), glucuronic acid (16–17%), nitrogen (0.327–0.365%) and protein (2.16–2.41%) contents of the gum arabic samples were consistent with the FAO (1990) specification for Acacia gum. In contrast the gum tahla samples had positive [α]_D values (+45° to +54°), lower rhamnose (3–4%) and higher arabinose (41–45%) contents and lower nitrogen (0.147–0.175%), and hence protein (0.97–1.15%), contents. All of the gum arabic samples precipitated with β‐glucosyl Yariv reagent and hence were shown to contain arabinogalactan‐protein(s) (AGPs), whereas in all but one of the gum tahla samples AGPs were not detected. The strong interaction of gum tahla with a monoclonal antibody known to recognize arabinose residues present in AGPs and arabinogalactans (AGs) was consistent with the observed higher levels of arabinose present in the gum tahla samples relative to the gum arabic samples. The data presented confirm that there are a number of physicochemical and structural differences between gum arabic (A. senegal gum) and gum tahla (A. seyal gum), and that a quick and simple immunological technique (immunodot blots) using an antiAGP/AG monoclonal antibody (MAC 207) could be used to screen for the presence of gum tahla in gum arabic consignments.

Keywords:

• gum arabic
• gum tahla
• Acacia Senegal
• Acacia seyal
• arabinogalactan‐protein

Notes

Dedicated to the memory of Gabriel B. J. Chapola.

To whom correspondence should be addressed: ConvaTec WHRI, First Avenue, Deeside Industrial Park, Clwyd CHS 2NU, UK.