The regulatory effects of L. plantarum peptidoglycan microspheres on innate and humoral immunity in mouse

Abstract

Objective: To study the effects of Lactobacillus Plantarum cell wall peptidoglycan (LPG) microspheres on mouse intestinal flora changes, peptidoglycan recognitions protein (PGRP) and cytokines expression levels.

Method: Plate counting was used for enumeration of the intestinal flora. Real-time PCR was used for quantification PGRP in different tissues. Cytokines content were determined by ELISA kits.

Result: The mouse administered orally with LPG microspheres showed significantly higher number of Lactobacillus and Bifidobacterium in caecum contents (p < 0.01). The amount of PGRP expression in different organs was highest in LPG microspheres-treated group. IL-4, 12, IFN-γ, TNF-α contents in serum from LPG microspheres-treated mouse were significantly higher than those in normal saline-treated group (p < 0.01).

Conclusions: This study shows that the LPG microspheres can regulate intestinal flora imbalance and improve systemic immunity, improve both Th1 and Th2 immune response, which provide some basis for the use of LPG as potential adjuvants.

Keywords:

• Peptidoglycan microspheres
• intestinal flora
• immune regulation
• PGRP

Acknowlegement

This work was supported by the basic scientific research business fees Heilongjiang University special funds item of Heilongjiang Province (HDJCCX-201612), the Natural Science Foundation of Heilongjiang Province (C2016049), Open item from Key Laboratory of Functional Inorganic Material Chemistry (Heilongjiang University), Ministry of Education and support programme from Biomass and Mineral Resources Efficient Use Collaborative Innovation Center R & D incubation and transformation. We would also give thanks to professor Haiying Li for providing RT-PCR apparatus use.

Disclosure statement

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the article.