Nanoencapsulation and characterisation of Hypericum perforatum for the treatment of neuropathic pain

Abstract

Aim

This work aimed to encapsulate Hypericum perforatum extract (HPE) into nanophytosomes (NPs) and assess the therapeutic efficacy of this nanocarrier in neuropathic pain induced by partial sciatic nerve ligation (PSNL).

Methods

Hydroalcoholic extract of Hypericum perforatum was prepared and encapsulated into NPs by thin layer hydration method. Particle size, zeta potential, TEM, differential scanning calorimetry (DSC), entrapment efficiency (%EE), and loading capacity (LC) of NPs were reported. The biochemical and histopathological examinations were measured in the sciatic nerve.

Results

Particle size, zeta potential, %EE, and LC were 104.7 ± 1.529 nm, −8.93 ± 1.71 mV, 87.23 ± 1.3%, and 53.12 ± 1.7%, respectively. TEM revealed well-formed and distinct vesicles. NPHPE (NPs of HPE) was significantly more effective than HPE in reducing PSNL-inducing pain. Antioxidant levels and sciatic nerve histology were reversed to normal with NPHPE.

Conclusions

This study demonstrates that encapsulating HPE with phytosomes is an effective therapeutic approach for neuropathic pain.

Keywords:

• Nanophytosomes
• Hypericum perforatum
• neuropathic pain
• sciatic nerve
• antioxidants

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

The financial support from the Dr. A.P.J Abdul Kalam Technical University, Lucknow, Uttar Pradesh [Grant No. Dr. APJAKTU/VRPS-2020/5751] was gratefully acknowledged.