Optimisation and in-vivo evaluation of extracted Karanjin loaded liposomal topical formulation for treatment of psoriasis in tape-stripped mouse model

Abstract

Aim

The present work is focus on development of anti-psoriasis activity of Karanjin (isolated from Pongamia pinnata seed oil) loaded liposome based lotion for enhancement of skin permeation and retention.

Method

Karanjin was isolated using liquid-liquid extraction method and characterised by HPLC analysis and partition coefficient. Further, isolated Karanjin was loaded into liposomes using thin-film hydration technique and optimised by Box-Behnken design. Selected optimised batch was characterised their mean diameter, PDI, zeta potential, and entrapment efficiency, morphology (by TEM), FTIR and ex-vivo skin retention. Additionally, Karanjin loaded liposomes were formulated into lotion and characterise their rheological, spreadability, texture, ex-vivo skin permeation & retention, stability and anti-psoriatic activity in mouse tail model.

Result

The yield of Karanjin from seed oil was 0.1% w/v and have lipophilic nature. The optimised liposomal formulation showed 195 ± 1.8 nm mean diameter, 0.271 ± 0.02 PDI, −27.0 ± 2.1 mV zeta potential and 61.97 ± 2.5% EE. TEM image revel the spherical shap of liposome surrounded by single phospholipid bilayer and no interection between drug and excipients. Further, lotion was prepared by 0.1% w/v carbopol and found to 615 mPa.sec viscosity, good thixotropic behaviour, spreadability and texture. There was 22.44% increase in drug permeation for Karanjin loaded liposomal lotion compared to pure Karanjin lotion, confirm by ex-vivo permeation and retention. While, in-vivo study revel the liposomal lotion of Karanjin was found to have 16.09% higher drug activity then 5% w/w conventional Karanjin lotion.

Conclusion

Karanjin loaded liposomal lotion have an effective anti-psoriatic agent and showed better skin permeation and retention than the conventional Karanjin lotion.

Keywords:

• Karanjin
• liposomal lotion
• skin irritation
• psoriasis
• Pongamia pinnata

Acknowledgement

Authors are thankful to Phospholipid GmbH, Nattermannalle, Germany for providing gift sample of Phospholipid 90H. Authors would also like to thank Mr. Amit Kumar, animal house facility, Shoolini University, Solan, HP, India for providing necessary care to animals. All tools and database were used under supervision on Department. Prof. (Dr.) Perwez Alam acknowledge the support of the Researchers Supporting Project number (RSPD2024R945), King Saud University, Riyadh, Saudi Arabia. This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

Author contributions

AS: methodology and formulation development, conception of design, AA: conception of design, data acquisition and analysis, HKD: manuscript drafting and revising the manuscript, KS: formal analysis, final drafting, PA: resource person, data evaluation, DK: conception of design, data acquisition and analysis, revising the manuscript, supervisory support.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.