Separation of liquid crystalline racemic mixtures obtained on the basis of (R,S)-2-hexanol on amylose tris(3-chloro-5-methylphenylcarbamate) covalently immobilised on silica in high-performance liquid chromatography

ABSTRACT

The separation on the enantiomers of thirteen newly synthesised liquid crystalline racemic mixtures obtained on the basis of (R,S)-2-hexanol was studied in HPLC on a chiral column prepared by covalent immobilisation of the chiral selector, namely amylose tris(3-chloro-5-methylphenylcarbamate) on silica. The mobile phases (MPs) consisting of n-heptane/isopropanol (IPA) or acetonitrile (ACN)/water (H₂O) at different volume ratios were used. All measurements were made at room temperature. The order of the elution was also determined. The chromatographic parameters such as: resolution - (R), selectivity - (α) and number of theoretical plates - (N) were determined. On the basis of the obtained results, optimal conditions were indicated for the best separation of the tested racemic mixtures.

KEYWORDS:

• Polysaccharide-based chiral stationary phase
• racemate
• enantiomer
• chiral chromatography

Disclosure statement

No potential conflict of interest was reported by the author.

Supplemental data

Supplemental data for this article can be accessed online at https://doi.org/10.1080/02678292.2023.2221669.

Correction Statement

This article has been corrected with minor changes. These changes do not impact the academic content of the article.

Additional information

Funding

This work was supported by the National Science Center Grant – the Miniatura 5 entitled “Optimisation of the separation of liquid crystalline racemic mixtures on chiral columns by high performance liquid chromatography” [DEC-2021/05/X/ST4/0016] and University Research Grant [UGB 22-801].