Abstract
Sheep red blood cells, treated with glutaraldehyde and sensitized with a purified Paracoccidioides brasiliensis antigen of molecular mass 43 kDa (gp43), were used to detect antibodies to P. brasiliensis by passive haemagglutination (PHA). Sera from 50 patients with paracoccidioidomycosis had PHA titres of > 1/800, and had antibodies detectable by immunodiffusion while antibodies to gp43 were detectable by PHA in sera from patients with histoplasmosis, aspergillosis, candidiasis, Jorge Lobo's disease or healthy people, only at titres of <1/100. Considering a cut-off at I/200, the sensitivity and specificity of PHA for paracoccidioidomycosis was 100%. Thus, the PHA test could be an efficacious method for routine serological diagnosis of paracoccidioidomycosis, with results in 1 to 2 h.
Resumen
Células de glóbulos rojos de carnero, tratadas con glutaraldehido y sensibilizadas con un antígeno purificado de Paracoccidioides brasiliensis de massa molecular de 43 kDa (gp43) fueron usados para detectar anticuerpos para Paracoccidioides brasiliensis por hemoaglutinación pasiva. Suero desde 50 pacientes con paracoccidioidomicosis tuvierón títulos > 1/800, y anticuerpos dectados por inmunodifusion. Sueros de pacientes con histoplasmosis, aspergilosis, candidiasis, enfermidad de Jorge Lobo y personas sanas tuvieron titulos < 1/100. Por lo tanto, el test proporciona un método eficaz para el diagnóstico serológico rutinario de paracoccidioidomicosis, proporcionando resultados en 1 a 2 horas.