Molecular and morphological data reveal hidden diversity in common North American Frustulia species (Amphipleuraceae)

Abstract

Frustulia Rabenhorst is an established diatom genus that is common and widespread across North America. As for many diatom genera, taxa within Frustulia have been the subject of taxonomic confusion. Although recent studies have examined species from Europe and New Zealand, there exist no detailed genetic data for North American individuals. Using both molecular (rbcL and 18S rRNA sequences) and morphological (i.e., frustule characters and shape analysis) data, we investigated common taxa of Frustulia in North America. European and New Zealand taxa were also included to investigate how North American species were related. We recognized nine taxa from North America in this study including two unknowns. A new species, Frustulia gibsonea sp. nov. is described. Frustulia gibsonea was observed in previous studies and described as F. cf. krammeri based on morphology. The use of molecular characters in this study demonstrates that this taxon is a distinct species, and not a morph of Frustulia krammeri. Despite differences in rbcL and 18S, F. gibsonea and F. krammeri are quite similar morphologically, showing overlap with both traditional measurements and shape analysis. This suggests that the combination of molecular and morphological data can help in deciphering these morphologically cryptic taxa. Frustulia saxonica and F. krammeri could be weakly separated based on sequence divergences, although in this case morphological characters were more useful. As evidenced by the low sequence divergence values obtained between the two taxa, they are closely related. Future molecular research, focusing on less conserved markers, will be necessary to resolve the relationships of these taxonomic complexes.

Keywords:

• Frustulia
• rbcL
• 18S
• shape analysis
• morphology
• North America

Acknowledgements

We wish to extend our thanks to R. Bull for his direction and guidance in the DNA analysis and two reviewers for valuable comments and criticisms on the original submission. Collections from New York State (Department of Environment and Conservation) were under TRP permit. Collections from the Northwest Territories were under Aurora Research Institute licence numbers 14435 and 14965. Other collections from Canada (Ontario, Nova Scotia, Newfoundland) fall under a public access designation. No potential conflict of interest is here reported by the authors. Cette recherche a été financée par le Conseil de recherches en sciences naturelles et en génie du Canada, le Musée canadien de la nature, et le programme de Bourse d’études supérieures de l’Ontario (BÉSO).

Disclosure statement

No potential conflict of interest was reported by the authors.

ORCID

Andréanne J. Bouchard http://orcid.org/0000-0001-9448-6758

Amanda M. Savoie http://orcid.org/0000-0003-1701-9106

Supplemental data

Supplemental data for this article can be accessed at https://doi.org/10.1080/0269249X.2019.1704889.

Additional information

Funding

We acknowledge the support of the Natural Sciences and Engineering Research Council of Canada; Canadian Museum of Nature 2018–2021 (RAC); Government of Ontario; Fondation Baxter & Alma Ricard.