Protective Effect of Donepezil on Retinal Ganglion Cells In Vitro and In Vivo

Abstract

The neuroprotective effect of donepezil, an acetylcholinesterase inhibitor on retinal ganglion cells (RGCs), was examined. The survival of purified RGCs after exposure to glutamate with or without donepezil (10^{− 7} M to 10^{− 5} M) was measured after 3 days in culture. In vivo, the neuroprotective effect of donepezil was examined by quantifying the number of viable RGCs 7 days after axotomy in adult rats. In vitro, donepezil dose-dependently reduced RGC death caused by glutamate toxicity. Oral administration of donepezil (10 mg kg⁻¹ day⁻¹) significantly reduced RGC death after axotomy. In conclusion, donepezil exerts a protective effect on RGCs both in vitro and in vivo.

KEYWORDS:

• acetylcholinesterase inhibitor
• retinal ganglion cell
• optic nerve transection
• apoptosis
• neuroprotection