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Current Eye Research Volume 31, 2006 - Issue 2
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Original Article

Inhibition of Ocular Angiogenesis by Diced Small Interfering RNAs (siRNAs) Specific to Vascular Endothelial Growth Factor (VEGF)

Masatoshi Murata Mitake Eye Clinic, Morioka, Japan; Department of Biochemistry, School of Medicine, Iwate Medical University, Morioka, Japan
,
Takako Takanami Department of Biochemistry, School of Medicine, Iwate Medical University, Morioka, Japan
,
Shinji Shimizu Department of Biochemistry, School of Medicine, Iwate Medical University, Morioka, Japan
,
Yosiko Kubota Department of Biochemistry, School of Medicine, Iwate Medical University, Morioka, Japan
,
Saburo Horiuchi Department of Biochemistry, School of Medicine, Iwate Medical University, Morioka, Japan
,
Wataru Habano DNA Laboratory, School of Medicine, Iwate Medical University, Morioka, Japan
,
Jian-xing Ma Department of Medicine, University of Oklahoma Health Science Center, Oklahoma City, Oklahoma, USA
&
Sanai Sato Department of Medicine, University of Oklahoma Health Science Center, Oklahoma City, Oklahoma, USA
 show all
Pages 171-180 | Received 01 Mar 2005, Accepted 04 Sep 2005, Published online: 02 Jul 2009
 
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Abstract

The effects of diced small interfering RNAs (siRNAs) designed for vascular endothelial growth factor (VEGF) on the expression of VEGF in human retinal pigment epithelial cell line ARPE-19 cells in vitro and on corneal angiogenesis in vivo were examined. The exposure to diced siRNAs significantly reduced the VEGF mRNA expression in ARPE-19 cells with minimal toxicity. In suture-induced corneal angiogenesis models, diced siRNAs minimized the severity of angiogenesis. Histological analysis displayed no particular tissue damage in the conjunctiva where siRNA was injected. The approach using diced siRNAs can be a new tool for various neovascular ocular diseases.

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