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Current Eye Research Volume 33, 2008 - Issue 4
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Original Article

Femtosecond Laser-Assisted Retinal Imaging and Ablation: Experimental Pilot Study

Matthias Hild Department of Ophthalmology, Saarland University Hospital, Homburg, Germany
,
Matthias Krause Department of Ophthalmology, Saarland University Hospital, Homburg, Germany
,
Iris Riemann Fraunhofer IBMT Institute of Biomedical Engineering, St. Ingbert, Germany
,
Pedro Mestres Department of Anatomy and Cell Biology, University of Saarland, Homburg, Germany
,
Sergey Toropygin Department of Ophthalmology, Tver State Medical Academy, Tver, Russia
,
Ursula Löw Department of Ophthalmology, Saarland University Hospital, Homburg, Germany
,
Karin Brückner Department of Ophthalmology, Saarland University Hospital, Homburg, Germany
,
Berthold Seitz Department of Ophthalmology, Saarland University Hospital, Homburg, Germany
,
Christian Jonescu-Cuypers Department of Ophthalmology, Saarland University Hospital, Homburg, Germany
&
Karsten König Fraunhofer IBMT Institute of Biomedical Engineering, St. Ingbert, Germany
 show all
Pages 351-363 | Received 08 Oct 2007, Accepted 29 Jan 2008, Published online: 02 Jul 2009
 
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Abstract

Purpose: To investigate retinal imaging and ablation using femtosecond laser pulses. Materials and Methods: Two non-amplified near-infrared femtosecond lasers were used to irradiate porcine retinal specimens in vitro. The lasers were used for tissue removal as well as multiphoton laser scanning microscopy. Results: Ablation of the nerve fiber layer was performed at pulse energies of 1.0 nJ to 3.9 nJ. Control laser scanning images were acquired within seconds after irradiation. Specimens were additionally investigated with electron microscopy. Conclusions: Non-amplified femtosecond lasers may allow precise surgery controlled by fast high-resolution imaging of the target.

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