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Current Eye Research Volume 33, 2008 - Issue 5-6
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Original Article

Dexamethasone Increases Pigment Epithelium-Derived Factor in Perfused Human Eyes

Elizabeth M. Perruccio Section of Protein Structure and Function, Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, NIH, Bethesda, Maryland, USA
,
Laura Leigh S. Rowlette Duke University Medical Center, Durham, North Carolina, USA
,
Núria Comes Department of Ophthalmology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
,
Silvia Locatelli-Hoops Section of Protein Structure and Function, Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, NIH, Bethesda, Maryland, USA
,
Luigi Notari Section of Protein Structure and Function, Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, NIH, Bethesda, Maryland, USA
,
S. Patricia Becerra Section of Protein Structure and Function, Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, NIH, Bethesda, Maryland, USA
&
Teresa Borrás Department of Ophthalmology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
 show all
Pages 507-515 | Received 11 Oct 2007, Accepted 06 Apr 2008, Published online: 02 Jul 2009
 
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Abstract

Purpose: To investigate the effects of dexamethasone (DEX) on pigment epithelium-derived factor (PEDF) cDNA and secreted protein in human trabecular meshwork (TM). Methods: Anterior segment organ cultures were perfused with 0.1 μ M DEX (OD) and vehicle (OS). Primary human TM cells (HTM) were treated with DEX under similar conditions. PEDF mRNA and secreted PEDF protein were quantitated by RT-PCR and Western blot. Results: PEDF mRNA and secreted PEDF protein levels were significantly higher in DEX over vehicle-treated cultures. In contrast, DEX decreased the activity of a 92-kDa gelatinolytic zymogen in organ culture effluents. Conclusion: DEX action in the human TM might include a PEDF-mediating pathway.

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