A Novel Vector System for Gene Transfer into the Cornea Using a Partially Dried Plasmid Expressing 18 Basic Fibroblast Growth Factor–Synthetic Amphiphile INTeraction-18 (SAINT-18) Complex

Abstract

Purpose: We describe a novel vector system of nonviral gene transfer into the cornea using a partially dried form of a plasmid expressing 18-kDa basic fibroblast growth factor (p-bFGF)–synthetic amphiphile INTeraction-18 (SAINT-18) complex. Methods: Corneal neovascularization (NV) was evaluated in 48 eyes of Sprague-Dawley rats after implantation of SAINT-18 containing 2 μ g of plasmid-expressing green fluorescent protein (p-GFP; control group), 0.2 μ g, 2 μ g, or 20 μ g of p-bFGF from day 0 to day 60. bFGF protein expression was analyzed by Western blotting and immunohistochemistry. Results: The p-bFGF–SAINT-18 complex induced dose-dependent corneal neovascularization, which reached a maximum on days 15–21 in the 20-μ g p-bFGF group, days 12–18 in the 2-μ g p-bFGF group, and on days 9–15 in the 0.2-μ g p-bFGF group, and then regressed progressively. No NV was observed in the p-GFP group. Conclusions: This noninflammatory corneal transfection model using partially dried p-bFGF–SAINT-18 complex allows precise localization of tranfection reagents for producing corneal neovascularization.

KEYWORDS:

• angiogenesis
• cornea
• fibroblast growth factor
• nonviral vector
• SAINT