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Retina

Increased Expression of IL-23 Receptor (IL-23R) in Vogt–Koyanagi–Harada (VKH) Disease

, , , , &
Pages 1369-1373 | Received 26 Feb 2018, Accepted 04 Jun 2018, Published online: 18 Jul 2018
 

ABSTRACT

Objective: Vogt–Koyanagi–Harada (VKH) disease is an autoimmune disease mediated by T cells that target melanocytes. It has been shown that IL-23 receptor (IL-23R) signaling promotes the generation of pathogenic T helper 17 cells. The aim of this study was designed to detect the possible role of IL-23R in VKH disease.

Methods: Subjects were divided into an active and inactive VKH patient group and a normal control group. The IL-23R level in peripheral blood mononuclear cells (PBMCs) was measured by flow cytometry and real-time polymerase chain reaction. PBMCs were stimulated with serum from patients or controls to detect the influence of serum from VKH patients on IL-23R expression.

Results: The IL-23R mRNA level was markedly increased in PBMCs from the active VKH patient group as compared to normal controls. Flow cytometry analysis showed that there was also an elevated IL-23R protein level in PBMCs in active VKH patients. The IL-23R protein level was higher in PBMCs obtained from healthy controls when they were cultured with serum from active VKH patient as compared to cell cultured with serum from normal controls. After the intraocular inflammation in VKH patients was controlled, the IL-23R gene expression returned back to normal levels.

Conclusion: Our study suggests that an elevated IL-23R level may participate in the development of VKH disease.

Acknowledgment

We would like to thank all the donors who participated in the present study.

Competing interests

None of the authors has a proprietary or financial interest in any product mentioned.

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this paper.

Additional information

Funding

This work was supported by National Key R&D Program of China (2016YFC0904000), Natural Science Foundation Major International (Regional) Joint Research Project (81720108009), Chongqing Key Laboratory of Ophthalmology (CSTC, 2008CA5003), Chongqing Science & Technology Platform and Base Construction Program (cstc2014pt-sy10002) and the Natural Science Foundation Project of Chongqing (cstc2017shmsA130073).

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