ABSTRACT
Aim: To discuss the immunological mechanism in electroacupuncture (EA) treatment of dry eye syndrome (DES) by targeting the changes in conjunctival cytokine expression profile.
Method: Eligible DES patients were randomized into an EA group (EAG) or an acupuncture group (AG). The ocular surface disease index (OSDI), amount of tear production, and tear film break-up time (BUT) were observed to evaluate the efficacy. Conjunctival cells were collected from both effective and invalid cases to observe the expressions of cytokines by protein microarray. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were used for functional cluster and signaling pathway analysis of the differentially expressed proteins. Enzyme-linked immunosorbent assay (ELISA) was used to verify the specific differential proteins.
Result: After treatment, OSDI dropped and BUT extended in both groups, and the tear production increased only in the EAG (all P < .01). Compared with the AG, the improvement in tear production was more significant in the EAG (P < .01). There were 17 differentially expressed conjunctival cytokines between the effective and invalid cases in the EAG, and those expressed higher than the limit of detection (LOD) included monocyte chemoattractant protein 1 (MCP-1), macrophage colony-stimulating factor (M-CSF), regulated on activation in normal T-cell expressed and secreted (RANTES) and tissue inhibitor of metalloproteinases 1 (TIMP-1). GO analysis showed that the differential cytokines were mainly involved in cellular interaction, signaling pathways and reactions to stimuli. KEGG analysis revealed that the signaling pathways of these cytokines were mainly responsible for interactions between cytokines or between cytokines and their receptors, such as Jak-STAT signaling pathway, chemokine signaling pathway, and tumor necrosis factor signaling pathway.
Conclusion: EA can effectively treat DES by improving the symptoms, increasing tear secretion and extending BUT, which is possibly related to its regulation on the conjunctival cytokine expressions.
Acknowledgments
The authors thank Shanghai H-Wayen Biomedical Technology Co., Ltd.for detection of the protein microarray.
Author contribution
Zhang Dan, Zhao Yan, and Yang Yan-Ting equally contributed to this work. Zhang Dan, Zhao Yan, Liu Xiao-Xu, Yang Yan-Ting, and Ma Xiao-Peng were in charge of treatment, index detection and sample collection. Wu Dan-Yan and Zhao Yue finished analyzing the data of this trial. Ma Xiao-Peng designed the trial and Shi Zheng supervised this study. Zhang Dan wrote the manuscript and Hong Jue and Liu Jie revised it. All authors approved the final version of this article, including the author list.
Supplementary material
Supplemental data for this article can be accessed publisher’s website.