Lack of Association between Monocyte Chemoattractant Protein-1 (MCP-1) Gene Promoter Polymorphism and Behcet’s Disease with and without Ocular Involvement in Iranian Population: A Case–Control Study

ABSTRACT

Purpose

This case-control study aimed to evaluate the possible association of MCP-1 − 2518A/G genetic polymorphism with Behcet’s disease (BD) in the Iranian patients.

Materials and methods

This study was performed in 135 Behcet’s patients (51 ocular and 84 non-ocular) and 79 healthy individuals. Peripheral blood samples were genotyped for MCP-1 − 2518A/G using the PCR-RFLP technique.

Results

The statistical analysis of MCP-1 − 2518A/G showed no significant differences in genotype/allele frequencies between Behcet’s patients and controls. There was no significant association in genotype/allele frequencies between either ocular or non-ocular BD patients and controls. Also, different genotype/allele frequencies between ocular and non-ocular BD were not statistically significant.

Conclusions

In this study, with a threshold P-value of 0.05 and an estimated power of 0.81 to detect a significant association (odds ratio ≥1.2), we did not observe any association of this variant with Behcet's disease.

KEYWORDS:

• Behcet’s disease
• ocular involvement
• MCP-1 gene
• CCL2 gene
• -2518A/G polymorphism
• PCR-RFLP
• Iran

Availability of data and materials

All data generated or analysed during this study are included in this published article.

Consent for publication

Informed consent was obtained from all individual participants included in the study.

Disclosure statement

The authors declare that they have no competing interests.

Ethics approval and consent to participate

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. This case-control study has been approved by ethical committee of ophthalmic research center, Shahid Beheshti University of Medical Sciences (IR.SBMU.ORC.REC.1396.14).

Additional information

Funding

This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sector.