Abstract
Purpose
Moxifloxacin (MOX) is a fourth-generation fluoroquinolone and a broad spectrum antibiotic used in the management of bacterial keratitis (BK). This investigation aimed to formulate MOX-loaded chitosan/pectin cationic polyelectrolyte nanocapsules (CPNCs) for the effective topical treatment of BK.
Methods
Physicochemical properties like nanocapsule size, charge, drug entrapment efficiency (EE), viscosity, pH, and in-vitro release profile of CPNCs were evaluated. The in-vitro antibacterial activity of CPNCs and marketed formulations (MFs) was studied against Staphylococcus aureus. Ex-vivo corneal permeation studies of CPNCs were evaluated with the help of a modified diffusion apparatus, which was used with goat cornea. The pharmacodynamic study was performed with optimized CPNCs on a BK-induced rabbit eye model and compared with MF.
Results
The optimized nanocapsules appeared as positive charge (+19.91 ± 0.66) with a nano size (242.0 ± 0.30 nm) as calculated by the dynamic light scattering method. The in-vitro release profile of CPNCs exhibited sustained release properties. The ex-vivo permeation pattern also supported the improved drug permeation through the cornea from CPNCs as compared with MF. Draize irritation studies confirmed that the prepared formulation is compatible with the corneal tissue. The in-vivo study concluded that the antibacterial activity of CPNCs was improved when evaluated with MF.
Conclusion
The obtained results showed that CPNCs were the better choice for the management of BK therapy due to its capability to improve the corneal adhesion of CPNCs through direct interaction with the mucous membrane of the corneal tissue.
Acknowledgments
The author, Mr. Parasuraman Mohan, acknowledges Guru Ghasidas Vishwavidyalaya (A Central University), Bilaspur, Chhattisgarh, India, for granting a research fellowship to perform this research study. The researchers are incredibly thankful to the Department of University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh, India, for characterizationizing the particle size and charge of nanocapsules. The researchers were indebted to the Department of Anatomy, AIIMS, New Delhi, India, for granting permission to analyze the TEM samples. We profusely thank the Department of Biotechnology, Guru Ghasidas University, Bilaspur, for performing the prepared nanocapsules’ antimicrobial efficacy.
Disclosure statement
No potential conflict of interest was reported by the author(s).
Data availability statement
The data that support the findings of this study are available from the corresponding author, KK, upon reasonable request.