Green synthesis of silver nanoparticles using Macrolepiota procera extract and investigation of their HSP27, HSP70, and HSP90 inhibitory potentials in human cancer cells

Abstract

In this study, silver nanoparticles (AgNPs) were synthesized by a microwave-assisted green chemistry method using a crude extract of Macrolepiota procera mushroom. The synthesized M. procera-AgNPs (MP-AgNPs) were characterized by UV–VIS spectroscopy, fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), dynamic light scattering (DLS) technique, and scanning transmission electron microscopy (S-TEM). The cytotoxic activity of synthesized MP-AgNPs was investigated in human breast cancer (MCF-7), lung cancer (A549), osteosarcoma (Saos-2), and colon cancer (HT-29) cell lines. HSP27, HSP70, and HSP90 inhibitory potential of MP-AgNPs were determined in cancer cells by RT-PCR and Western blotting experiments. To analyze the apoptotic profile of MP-AgNPs in cancer cell lines, the expression levels of apoptotic and anti-apoptotic genes were determined using RT-PCR. The results showed that MP-AgNPs were exhibited anticancer activity in MCF-7, A549, Saos-2, and HT-29 cell lines. MP-AgNPs decreased gene and protein expression levels of HSP27, HSP70, and HSP90 in cancer cells. Also, MP-AgNPs induced apoptotic cell death mechanisms in MCF-7, A549, Saos-2, and HT-29 cell lines. MP-AgNPs are found to be significant metallic nanoparticle systems as efficient HSP inhibitors in the treatment of cancer.

Keywords:

• Silver nanoparticle
• cancer
• apoptosis
• heat shock proteins

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was supported by grants from Tokat Gaziosmanpaşa University, Foundation of Scientific Researches Projects (Project number: 2020/104).