Assessment of phenol-degrading ability of Acinetobacter sp. B9 for its application in bioremediation of phenol-contaminated industrial effluents

Abstract

Successful bioremediation of a phenol-contaminated environment requires application of those microbial strains that have acquired phenol tolerance and phenol-degrading abilities. A newly isolated strain B9 of Acinetobacter sp. was adapted to a high phenol concentration by growing sequentially from low- to high-strength phenol. The acclimatised strain was able to grow and completely degrade up to 14 mM of phenol in 136 h. The degradation rates were found to increase with an increase in the phenol concentration from 2.0 to 7.5 mM. The strain preferred neutral to alkaline pH range for growth and phenol degradation, with the optimum being pH 8.0. The optimum temperature for phenol degradation was found to be in the range of 30–35°C. Transmission electron micrographs showed a disorganised and convoluted cell membrane in the case of phenol-stressed cells, showing a major effect of phenol on the membrane. Enzymatic and gas chromatography-mass spectrometry studies show the presence of an ortho-cleavage pathway for phenol degradation. Efficient phenol degradation was observed even in the presence of pyridine and heavy metals as co-toxicants showing the potential of strain in bioremediation of industrial wastes. Application of strain B9 to real tannery wastewater showed 100% removal of initial 0.5 mM phenol within 48 h of treatment.

Keywords:

• dephenolisation
• Acinetobacter sp. B9
• bioremediation
• tannery effluent
• co-contaminants

Acknowledgements

We acknowledge the All India Institute of Medical Sciences (AIIMS), New Delhi, for the TEM facility.

Disclosure statement

No potential conflict of interest was reported by the authors.

Funding

The financial support provided by University Grants Commission (UGC), Govt. of India, in the form of a Senior Research Fellowship (SRF) to AB is gratefully acknowledged.

Supplemental data

Supplemental data for this article can be accessed at doi: 10.1080/02757540.2015.1075517.