79
Views
1
CrossRef citations to date
0
Altmetric
Original Articles

A rapid determination of histamine and 3-methylhistamine in human plasma by high performance liquid chromatography

&
Pages 787-793 | Received 03 Oct 2007, Accepted 06 Nov 2007, Published online: 20 Oct 2008
 

Abstract

Histamine plays an important pathophysiological role in allergy, inflammation, gastric acid secretion, microcirculation and neurotransmission. 3-Methylhistamine is a prominent metabolite of histamine. Different methods for determination of histamine in biological fluids have been developed. In the present study, a simple, simultaneous determination of histamine and 3-methylhistamine by HPLC (precolumn derivatization with fluorescamine) was developed in human plasma using fluorescence detection with 1-methylhistamine as the internal standard. Linear regression analysis of the ratios of the concentrations of histamine and 3-methylhistamine (X) against peak height ratios (Y) yielded the following: y = 0.0073x − 0.0096 (R 2 = 0.990) and y = 0.0077x − 0.0111 (R 2 = 0.989). In conclusion, it was possible to detect histamine and 3-methylhistamine below 5 ng mL−1 in 1 mL plasma.

Log in via your institution

Log in to Taylor & Francis Online

PDF download + Online access

  • 48 hours access to article PDF & online version
  • Article PDF can be downloaded
  • Article PDF can be printed
USD 61.00 Add to cart

Issue Purchase

  • 30 days online access to complete issue
  • Article PDFs can be downloaded
  • Article PDFs can be printed
USD 2,970.00 Add to cart

* Local tax will be added as applicable

Related Research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.