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Abstract
The molecular mechanisms underlying proliferation and apoptosis induced by p-nonylphenol (NP) through regulation of mRNA and protein expression of c-myc, p53 mRNA and the protein was investigated using ovarian cancer PEO4 cells. PEO4 cells were grown in the Dulbecco's modified Eagle medium (DMEM), 3(4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT) assay was employed to investigate cell proliferation. RT-PCR and immunohistochemistry was performed to determine mRNA and protein expression of PEO4 c-myc and p53. Within the range of approximately 8–9×10−7 mol L−1, NP stimulated proliferation in PEO4 cells in a concentration-and time-dependent manner. RT-PCR and immunohistochemistry analyses revealed that treatment with 32 × 10−7 mol L−1 NP for 72 hr resulted in increased mRNA and protein expression of PEO4 c-myc and p53. Evidence indicates that NP exerted estrogenic actions and stimulated proliferation in estrogen responsive ovarian cancer PEO4 cells by up-regulation of c-myc mRNA and protein expression.