76
Views
1
CrossRef citations to date
0
Altmetric
Eco/Toxicology

Gutkha chewing induces oxidative disturbances and cellular DNA damage in humans

, , &
Pages 388-402 | Received 02 Oct 2011, Accepted 08 Nov 2011, Published online: 06 Dec 2011
 

Abstract

Chewing of gutkha is a risk factor for several diseases. The purpose of this study was to determine the effects of gutkha on oxidative damage and antioxidant enzyme status in serum, lymphocyte, and neutrophils. Male subjects were randomly selected from university students (21–24 years) in a rural community in the District of Midnapore, West Bengal, India. In order to examine oxidative damage and antioxidant enzyme status, superoxide radical generation (), nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity was measured in lymphocytes and neutrophils; and in serum, neutrophils, and lymphocytes the levels of lipid peroxidation (MDA), reduced glutathione (GSH), and oxidized glutathione (GSSG) as well as activities of lactate dehydrogenase (LDH), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione reductase (GR) were measured. Deoxyribonucleic acid (DNA) damage in lymphocytes and neutrophils was determined by diphenylamine (DPA) assay and 1.2% gel electrophoresis. There was a significant increase in generation, NADPH oxidase, LDH activity, and DPA in lymphocytes and neutrophils, and a rise in the levels of MDA and GSSG in serum, lymphocytes, and neutrophils of gutkha chewers. GSH levels, redox ratio, and the activity of SOD, CAT, GPx, and GR of serum, lymphocyte, and neutrophils in gutkha chewers were significantly decreased. Agarose gel electrophoresis showed a smeary pattern of DNA. These findings suggest that chewing of gutkha produced oxidative stress and impaired antioxidant defense system.

Acknowledgments

Conflict of interest: There are no conflicts of interests.

Log in via your institution

Log in to Taylor & Francis Online

PDF download + Online access

  • 48 hours access to article PDF & online version
  • Article PDF can be downloaded
  • Article PDF can be printed
USD 61.00 Add to cart

Issue Purchase

  • 30 days online access to complete issue
  • Article PDFs can be downloaded
  • Article PDFs can be printed
USD 2,970.00 Add to cart

* Local tax will be added as applicable

Related Research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.