Effects of copper on lipid peroxidation, glutathione, metallothionein, and antioxidative enzymes in the freshwater mussel Anodonta anatina

Abstract

Copper is an essential element to all animals. At elevated concentrations, it is toxic and can participate in the formation of reactive oxygen species, leading to cellular damage. In this study, the ecotoxicological relevance of copper was investigated with freshwater mussels, Anodonta anatina. When the mussels were exposed to copper at environmentally realistic concentrations, either via the water (0.3 µmol L⁻¹ Cu) or fed with Cu-loaded algae (equivalent to 0.06 µmol L⁻¹ Cu), the level of thiobarbituric acid-reactive substances rose and glutathione decreased. This was associated with the induction of metallothionein and, relative to total protein, of glutathione reductase and the antioxidative enzymes superoxide dismutase, catalase, and glutathione peroxidase. But, since the overall protein-synthetic capacity was hampered by the copper insult, the activities of the enzymes relative to tissue weight and copper concentrations were depressed. During depuration, most parameters started to normalize although not returning to control values within 12 days.

Keywords:

• copper
• Anodonta anatina
• thiobarbituric acid-reactive substances
• glutathione
• metallothionein
• antioxidative enzymes

Acknowledgments

Financial support by Directorate General of Higher Education, Ministry of National Education of the Republic of Indonesia, is highly appreciated.