Nanoparticles influence on expression of cell cycle related genes in Drosophila: a microarray-based toxicogenomics study

Abstract

Relatively little is known regarding the interaction of nanoscale objects with dynamic complex biological systems. Microarray-based toxicogenomics studies may serve as a suitable technique to explore the genome wide effects of nanoparticles on any organism through a single experiment. The influence of nanoporous aluminosilicate nanoparticles (NP), citrate-capped gold NP, lipophilic silica NP, BSA-capped silver NP, and lipophilic zinc oxide NP were studied on 75 cell cycle-related genes of adult Drosophila melanogaster. Microarray experiments were conducted after the flies were fed with NP-mixed media for 15 days. Data showed that silver, zinc oxide, and alumino silicate NP predominantly perturbed cell cycle genes, whereas gold and silica NP exerted the least influence on these genes.

Keywords:

• nanoparticle
• toxicogenomics
• Drosophila
• cell cycle

Acknowledgments

We thank Department of Biotechnology, Govt. of India, (DBT) (grant nos- BT/PR9050/NNT/28/21/2007 & BT/PR8931/NNT/28/07/2007) and NAIP, ICAR (grant no- NAIP/Comp-4/C3004/2008-09) for their generous financial support. ISI plan project for 2008–2011 was also used for funding this work.