Microbiological evaluation of 4-(6-chloro-7H-purin-8-ylamino)-3-nitro-chromen-2-one with a comparison to antibacterial activity of standard drugs

Abstract

Nucleophilic substitution of 4-chloro-3-nitro-2-oxo-2H-chromen-2-one 3 by 6-chloro-7H-purin-2-ylamine (a), yielded the corresponding 4-(6-chloro-7H-purin-8-ylamino)-3-nitro-chromen-2-one 4. The chemical structure of synthesized compound was characterized using IR and NMR spectra, and elemental analyses. The purified synthesized compound 4 was tested at concentrations 1, 3, 5 mg/ml for its antibacterial activity against three bacterial cultures: Staphylococcus aureus, Escherichia coli, and Bacillus cereus. The antibacterial activity of synthesized compound was compared to antibacterial activity of standard antibiotics cephalexine and streptomycin.

Keywords:

• 4-(6-chloro-7H-purin-8-ylamino)-3-nitro-chromen-2-one synthesis
• antibacterial activity
• Staphylococcus aureus
• Escherichia coli
• Bacillus cereus
• cephalexine
• streptomycin

Acknowledgment

We thank Ministry for Science and Technology of the Republic of Kosovo for partial support through a grant and dr Shuhreta Omeragiq from the Microbiological Laboratory of Regional Water Company, Prishtina, Republic of Kosovo for antimicrobial testing of the compound prepared.