In vitro anti-inflammatory and free radical scavenging properties of Oldenlandia auricularia

Abstract

The in vitro anti-inflammatory and antioxidant properties of extract of Oldenlandia auricularia with 70% aqueous alcohol have been evaluated. The inhibition of nuclear translocation of nuclear factor kappa-light-chain-enhancer of activated B cells in lipopolysaccharide stimulated murine macrophage cells was evaluated. Messenger ribonucleic acid levels of tumor necrosis factor-α and cyclooxygenase-2 were analysed by quantitative real time polymerase chain reaction. O. auricularia (100 mg/L) showed better anti-inflammatory and antioxidant activities when compared to its lower doses, whereas the standard drugs curcumin and diclofenac sodium showed significant anti-inflammatory and antioxidant potential. Treatment at this concentration inhibited the signalling pathway of nuclear factor kappa-light-chain-enhancer of activated B cells and messenger ribonucleic acid expression of tumor necrosis factor- α, and cyclooxygenase-2. Liquid chromatography/quadrupole time-of-flight mass spectrometry revealed the presence of previously reported compounds auricularine, ursolic acid and β-sitosterol. The observed anti-inflammatory property of O. auricularia could be due to the inhibition of nuclear factor kappa-light-chain-enhancer of activated B cells p65 sub unit nuclear translocation and inhibition of its signalling pathway.

Keywords:

• Oldenlandia auricularia
• nuclear factor kappa-light-chain-enhancer B cells
• nuclear translocation
• tumour necrosis factor-α
• cyclooxygenase-2

Acknowledgments

The authors thankfully acknowledge The Director, JNTBGRI for the facilities provided.

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Funding

This work was supported by Science and Engineering Research Board (SERB), Department of Science and Technology (DST), Government of India. Fast Track Young Scientist Scheme awarded to Dr. G. I. Anuja (No.YSS/2014/000411).