Use of rat embryo limb bud cell cultures to screen organochlorine compounds detected in the water and sediment of rivers in Tokyo metropolis for developmental toxicity

Abstract

Developmental toxicity of nine organochlorine compounds commonly detected in the water and sediment of rivers in the Tokyo metropolitan area was screened by rat embryo limb bud cell culture (LBC). The chemicals were p‐dichlorobenzene (pDCB), o‐dichlorobenzene (oDCB), p‐chloroaniline (pCA), 3,4‐dichloroaniline (DCA), tris (2‐chloroethyl)phosphate, 2,5‐dichlorophenol (DP), 2,5‐dichloro‐anisole (DA), Triclosan (IR) and Triclocarban (TC).

The ID₅₀ (50% inhibitory concentration for differentiation) and the IP₅₀ (50% inhibitory concentration for proliferation) of pCA, IR and TC, and the ID₅₀ of DP were low among the organochlorine compounds tested here. The P/D ratio (IP₅₀/ID₅₀) of pCA and DP was 2.95 and >6.1, respectively. The ID₅₀ and the IP₅₀ of DCA, pDCB, oDCB and DA were submillimolar level, and their P/D ratios were around 1. Although the ID₅₀ and the IP₅₀ of TCEP were relatively high (low toxicity), its P/D ratio was relatively high among the organochlorine compounds tested here.

Since DP had a very high P/D ratio, pCA and TCEP are carcinogens and had relatively high P/D ratios, these three compounds have priority for further investigation of developmental toxicity.

Keywords:

• Organochlorine compounds
• Tokyo rivers
• Developmental Toxicity
• Limb bud cell cultures

Notes

Corresponding Author