The potential role of mechanosensitive ion channels in substrate stiffness-regulated Ca²⁺ response in chondrocytes

ABSTRACT

Purpose

The stiffness of the pericellular matrix (PCM) decreases in the most common degenerative joint disease, osteoarthritis (OA). This study was undertaken to explore the potential functional role of transient receptor potential vanilloid 4 (TRPV4), Piezo1, and Piezo2 in transducing different PCM stiffness in chondrocytes.

Methods and Results

Polydimethylsiloxane (PDMS) substrates with different stiffness (designated 197 kPa, 78 kPa, 54 kPa, or 2 kPa, respectively) were first prepared to simulate the decrease in stiffness of the PCM that chondrocytes encounter in osteoarthritic cartilage. Next, the TRPV4-, Piezo1-, or Piezo2-knockdown primary chondrocytes (designated TRPV4-KD, Piezo1-KD, or Piezo2-KD cells) were seeded onto these different PDMS substrates. Then, using a Ca²⁺-imaging system, substrate stiffness-regulated intracellular Ca²⁺ influx ([Ca²⁺]_i) in chondrocytes was examined to investigate the role of TRPV4, Piezo1, and Piezo2 in Ca²⁺ signaling in response to different stiffness. Results showed that the characteristics of intracellular [Ca²⁺]_i in chondrocytes regulated by PDMS substrate exhibited stiffness-dependent differences. Additionally, stiffness-evoked [Ca²⁺]_i changes were suppressed in TRPV4-KD, Piezo1-KD, or Piezo2-KD cells compared with control siRNA-treated cells, implying that any channel is fundamental for Ca²⁺ signaling induced by substrate stiffness. Furthermore, TRPV4-mediated Ca²⁺ signaling played a central role in the response of chondrocytes to 197 kPa and 78 kPa substrate, while Piezo1/2-mediated Ca²⁺ signaling played a central role in the response of chondrocytes to 54 kPa and 2 kPa substrate.

Conclusions

Collectively, these findings indicate that chondrocytes might perceive and distinguish the different PCM stiffness by using different mechanosensitive ion channels.

KEYWORDS:

• Chondrocyte
• pericellular matrix stiffness
• mechanosensitive ion channel
• calcium signaling

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This study was financially supported by the National Natural Science Foundation of China (grant no. 12102238,11872263,11632013,), Basic Research Program of Shanxi for Youths (201901D211322), and Science Research Start-up Fund for Doctor of Shanxi Medical University (XD1813).