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Abstract
Two nodaviruses, both isolated from New Zealand scarab beetles, replicated in DSIR-HA-1179 (HA) cells derived from the black beetle, Heteronychus arator (Coleoptera: Scarabaeidae). Black beetle virus and Flock House virus, isolated from the grass grub (Costelytra zealandica) produced a characteristic cytopathic effect in cells. Virus replication was demonstrated by showing viral RNA synthesis in infected cells. A comparison between HA cells and cells derived from Drosophila melanogaster (line 1) showed that the Drosophila cells were twice as sensitive for detecting black beetle virus and 5 times as sensitive for detecting Flock House virus. Replication of Flock House virus in Drosophila cells was more rapid and more productive than in HA cells.