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Original Articles

Rhopalosiphum aphids in New Zealand. I. RAPD markers reveal limited variability in lineages of Rhopalosiphum padi

, , , , &
Pages 29-36 | Received 12 Nov 2003, Accepted 13 Nov 2004, Published online: 30 Mar 2010
 

Abstract

Theoretical studies have suggested that New Zealand populations of Rhopalosiphum padi should consist of genetically separate lineages. We applied random amplified polymorphic DNA (RAPD) PCR to genetic material of Rhopalosiphum aphids from cereal crops and pastures. Several putative R. padi lineages were detected by RAPD PCR in 1995 samples. Subsequent RAPD analyses produced good evidence for the presence of distinct R. padi lineages in New Zealand but reduced the number of predominant lineages to two and revealed low overall genetic variability. One aphid grouping yielded substantially different RAPD patterns from the other aphids, suggesting the presence of a cryptic New Zealand Rhopalosiphum sp.

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