Adsorptive transfer stripping voltammetry applied to the study of the interaction between DNA and actinomycin D

Abstract

Actinomycin D is an antitumor antibiotic which intercalates within DNA. The interaction of actinomycin D with double-stranded and thermally denatured (single-stranded) calf thymus DNA was studied in solution as well as at the electrode surface using differential pulse transfer voltammetry with carbon paste electrode in 0.2 M acetate buffer, pH 5.0.

Keywords:

• Actinomycin D
• Adsorptive transfer stripping voltammetry
• DNA
• CPE
• Intercalator
• Anti-cancer drugs

Acknowledgements

Ioanna Ch. Gherghi wish to thank the Greek State Scholarship Foundation for financial support for her PhD studies.

The authors wish to thank the Laboratory of Biochemistry of the Aristotle University and particularly the Director, Prof. D. Kyriakidis, and the Lecturer, Dr A. Pantazaki, for initial discussions around the subject.