Strategy for the development of sensor platforms for multi-analysis

Abstract

Colloidal gold biofunctionalised nanomodules were synthesised and characterised by fluorescence and colorimetric measurements. These biofunctionalised nanomodules were selectively electrodeposited on electrode arrays. When the biomolecule used was an oligonucleotide probe, the gold conjugates were hybridised with complementary and mutated sequences, and the hybridisation efficiency was measured by amperometry. Significant differences in current density between complementary and mutated target sequences were achieved. Horseradish peroxidase-colloidal gold was also selectively electrodeposited on screen-printed electrodes providing proof-of-concept for a construction method of enzyme multi-sensors. Oligonucleotide and enzyme arrays were thus demonstrated, providing a generic platform for use in multi-analysis.

Keywords:

• Electrodeposition
• Array
• Colloidal gold
• Oligonucleotide
• Horseradish peroxidase (HRP)
• Biorecognition nanomodule
• Hybridisation

Acknowledgements

This article has been carried out with financial support from the Commission of the European Communities, specific RTD programme QOL, QLK2-CT-2000-00765, ‘DISSARM: Development of Integratable Sensors for Screening of Antibiotic Resistant Mycobacteria’. It does not necessarily reflect its views and in no way anticipates the Commission's future policy in this area. DINAMIC is an R&D and Technology Transfer centre dedicated to the support and encouragement of innovation in the private sector. DINAMIC is member of the network for the support of innovation and is partially funded by the ministry of the industry (CIDEM) of the autonomous government of Catalonia.