42
Views
6
CrossRef citations to date
0
Altmetric
Original Articles

Detection of DNA/DNA hybridization by electrogenerated chemiluminescence

Pages 609-612 | Received 08 Oct 2004, Accepted 07 Mar 2005, Published online: 25 Jan 2007
 

Abstract

Oligonucleotides labelled at 5′ with Ru(bpy)3 were hybridized to complementary DNA that had been previously immobilized on a glassy carbon electrode by anodic oxidation. The hybrids emitted detectable electrogenerated chemiluminescence upon application of 10–50 ms pulse(s) of 2.3 V. Pre-accumulation of the Ru(bpy)3 label at the electrode was found to enhance light emission as compared to the label in free solution. Since the intensity of the emitted light was greatly influenced by the electrode material, gold, platinum, and various carbon based materials (glassy carbon, graphite, carbon fibre, and composite plastic/carbon materials) were tested. The light generated at glassy carbon electrodes resulted at least two orders of magnitude higher than any other material tested.

Notes

Fax: 39-0432-558501. Email: [email protected]

Additional information

Notes on contributors

Giuseppe Firrao Footnote*

Fax: 39-0432-558501. Email: [email protected]

Log in via your institution

Log in to Taylor & Francis Online

PDF download + Online access

  • 48 hours access to article PDF & online version
  • Article PDF can be downloaded
  • Article PDF can be printed
USD 61.00 Add to cart

Issue Purchase

  • 30 days online access to complete issue
  • Article PDFs can be downloaded
  • Article PDFs can be printed
USD 1,223.00 Add to cart

* Local tax will be added as applicable

Related Research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.