Gold nanoparticle-based immunochromatographic assay for the detection of 7-aminoclonazepam in urine

Abstract

An analytical system of immunochromatographic assay based on gold nanoparticles was developed for the detection of 7-aminoclonazepam (7-ACLZ) in human urine. The qualitative assay was based on the competitive immunoassay using anti-7-ACLZ polyclonal antibody (PcAb) and a detector reagent that contains colloidal gold particles coated with anti-7-ACLZ PcAb. Nitrocellulose membrane was separately immobilised with goat anti-rabbit IgG (control line) and 7-ACLZ-OVA conjugate (test line). The sensitivity of the strip was tested for detecting 7-ACLZ spiked in urine and each specimen was independently measured by liquid chromatography tandem mass spectrometry. Good correlation was showed by the recovery results. The limit of detection for the strip test in urine was 100 ng mL⁻¹. The assay can be applied to the rapid detection of 7-ACLZ with the short testing time.

Keywords:

• 7-aminoclonazepam
• lateral flow
• gold-labelled immunochromatography
• LC/MS/MS

Acknowledgement

We greatly appreciate the financial support from National Natural Science Foundation of China (20675035).