276
Views
18
CrossRef citations to date
0
Altmetric
Original Articles

Monoclonal antibody-based enzyme-linked immunosorbent assay for detection of total malachite green and crystal violet residues in fishery products

, , , , , , & show all
Pages 959-969 | Received 13 Aug 2011, Accepted 13 Feb 2012, Published online: 15 May 2012
 

Abstract

A rapid easy-to-use trace level direct competitive enzyme-linked immunosorbent assay (dc-ELISA) detection of total residual malachite green (MG), crystal violet (CV) and their corresponding primary metabolites leucomalachite green (LMG) and leucocrystal violet (LCV) in fishery products in a single assay was developed. The monoclonal antibodies, anti-MG and anti-CV mAbs, were prepared using carboxyl-malachite green (CMG) and cationized bovine serum albumin (cBSA) conjugates as immunogen. The linear range for the quantitative detection of total MG, CV and their primary metabolites LMG and LCV was between 0.15 to 4.5 ng mL−1 with a half maximal inhibitory concentration (IC50) at 0.56 ± 0.04 ng mL−1 (n = 5). The anti-MG mAbs exhibited 98% cross-reactivity to CV, less than 0.1% cross-reactivity with LMG and LCV, and no cross-reactivity with chloramphenicol, enrofloxacin, sulfadiazine, and tetracycline. Application of the dc-ELISA in fish tissue samples gave a limit of detection (LOD) of 0.37 ng g−1. The improved total detection lead to a recovery of 74.60 ± 8.38% at 0.5 ng g−1 and 87.47 ± 12.83% at 2.0 ng g−1 that was better than existing techniques. The dc-ELISA showed total MG in 7 out of 44 field fish samples that were confirmed with LC-MS/MS. The easy-to-use, inexpensive, and rapid dc-ELISA for the detection of total MG, CV and their corresponding primary metabolites holds promise for field applications.

Acknowledgements

This work was supported by ‘Twelfth Five-Year Plan’ for National Science and Technology Support Program (2011BAK10B06, 2011BAK10B01, 2011BAK10B02), the work was also supported in part by the scientific research foundation for the returned overseas Chinese scholars (Z04925) and educational commission of Jiangxi Province, China (GJJ10009).

Log in via your institution

Log in to Taylor & Francis Online

PDF download + Online access

  • 48 hours access to article PDF & online version
  • Article PDF can be downloaded
  • Article PDF can be printed
USD 61.00 Add to cart

Issue Purchase

  • 30 days online access to complete issue
  • Article PDFs can be downloaded
  • Article PDFs can be printed
USD 1,223.00 Add to cart

* Local tax will be added as applicable

Related Research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.