Immobilised cytochrome c on the carbon dots functionalised MWCNTs and its application to hydrogen peroxide detection

ABSTRACT

We have prepared the carbon dots (C-dots) by sucrose carbonisation in the presence of sulphuric acid and characterised by transmission electron microscopy, Fourier transform infrared spectra, fluorescence spectroscopy and UV–vis absorption spectra. The resultant C-dots could be used for the activation of CNTs. Cytochrome c immobilised on the functionalised multi-walled carbon nanotube-modified glass carbon electrode was found to promote electron transfer that was investigated by electrochemical methods. The obtained electrode can also be used as a biocatalyser to catalyse the reduction of H₂O₂. Under optimal conditions, the constructed H₂O₂ sensor showed two linear segments and the detection limits were 1.02 and 2.59 µM (S/N = 3). The modified electrode displayed high stability, good reproducibility and excellent selectivity.

KEYWORDS:

• Cytochrome c
• carbon dots
• multi-walled carbon nanotubes
• hydrogen peroxide
• biosensors

Acknowledgements

This work is also supported by the National Natural Science Foundation of China: [Grant Numbers 21075001 and 20801001]. We also thank the Foundation for Innovation Team of Bioanalytical Chemistry for financial support.

Disclosure statement

No potential conflict of interest was reported by the authors.

supplemental data

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Additional information

Funding

This work was supported by the National Natural Science Foundation of China: [Grant Numbers 21075001 and 20801001].