Detection and assessment of risk factors associated with Newcastle disease virus infection in birds in backyard poultry in Laichau province of Vietnam

ABSTRACT

Newcastle disease (ND) is a highly pathogenic and contagious viral infectious disease of poultry that causes a very serious problem for poultry production and economic loss worldwide. ND has been an epizootic disease in Vietnam. Information about the risk factors that are associated with virus transmission in backyard chickens in Vietnam is limited. To provide more epidemiological information about ND in Vietnam, this study was performed to estimate NDV prevalence and identify the risk factors for ND virus (NDV) infection in birds at the backyard flock level. Choanal swabs were taken from 400 randomly selected birds from 100 apparently healthy flocks from May to July 2020. Based on RT–PCR analysis, 43 of 400 swab samples (10.75%; 95% CI 8–14.17) and 21 of 100 flocks (21%; 95% CI 14.17–29.98) were positive for the fusion (F) gene of NDV. The management practice risks were: backyard flocks contacting wild birds (OR = 3.89; P = 0.030), mixed flocks with different types and species of birds (OR = 5.46; P = 0.004), and infrequency of cleaning and disinfecting poultry houses (OR (odds ratio) = 4.43; P = 0.034). The second and third risks (above) showed a positive interaction on the risk of NDV infection in birds (OR = 39.38; P = 0.001), and the first risk showed a negative interaction. Further studies on NDV surveillance in domestic waterfowl, longitudinal studies, a well-optimized RT-qPCR assay, and genetic characterization are needed. The development of handbooks, flyers, or lessons for educating poultry keepers are also needed.

RESEARCH HIGHLIGHT

• RT–PCR was used to detect the F gene of NDV in choanal swabs.

• Risk factors associated with NDV-positive samples were determined.

• The evidence for NDV circulation in backyard healthy birds was observed.

• Contact with wild birds, mixed flocks, and poor hygiene were major risk factors.

KEYWORDS:

• Newcastle disease virus
• risk factors
• fusion gene
• backyard flock
• wild birds
• surveillance
• Vietnam

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by the Ministry of Agriculture and Rural Development of the Socialist Republic of Vietnam [grant number 5114/QĐ-BNN-KHCN].