BCMV-ukr: isolate of bean common mosaic virus revealed in Ukraine

SUMMARY

Bean common mosaic virus (BCMV) is distributed worldwide and causes a serious disease in bean reducing growth and crops yield. The aim of this study was to investigate the occurrence of BCMV and Bean common mosaic necrotic virus (BCMNV) in Ukraine, to characterise host range and reactions of indicator plants to mechanical inoculation with the isolate and to differentiate it by using Drijfhout’s differentials. Leaf samples were positive for BCMV infection in RT-PCR assay employing specific primers with amplification of a 340-bp product. Based on a biological test on bean differentials, the isolate was assigned to pathogroup VII despite the fact that strain differed markedly from the standard strains in symptoms producing on differential groups IV and V. Partial sequence data of the coat protein region show 100% identity with BCMV 125 sequences tested. To our knowledge, this is the first attempt to characterise the BCMV circulating in Ukraine.

Keywords:

• Bean common necrotic mosaic virus (BCNMV)
• polymerase chain reaction with reverse transcription (RT-PCR)
• indicator plants
• bean differentials

Additional information

Funding

This work was carried out predominantly with funds supplied by the National Academy of Sciences of Ukraine (governmental registration number of work is 0110U001871). Author Mishchenko Lidiya was supported by grant № 16БФ036-02 from Ministry of Education of Ukraine.