Abstract
To investigate the anti-TMV effects of Bunium persicum (BP) and Zataria multiflora (ZM), a set of experiments was conducted in a completely randomised design (n = 3) (p < 0.05). First, TMV particles were treated with different concentrations (0.5, 1.0, 2.5, and 5.0% (v/v)) of BP and ZM extracts. Nicotiana glutinosa and N. tabacum were mechanically inoculated with extract-treated TMV preparations at three intervals (24 h pre, simultaneous, and 24 h post-virus inoculation). Subsequently, local lesion number, inhibition rate, symptom index, and TMV accumulation were measured. Results showed that both extracts displayed significant anti-TMV effects. The highest inhibition percentages for BP and ZM extracts were 65.5% and 52.06%, respectively. 24 h pre-virus inoculation of both extracts at 5% was most efficient in reducing symptom index and ELISA extinction value. Therefore, ZM and BP can be considered as novel anti-TMV plants. Further studies are needed to find their mechanisms and evaluate their antiviral effects against other plant viruses.
Disclosure statement
No potential conflict of interest was reported by the author(s).