Talaromyces omanensis and Aspergillus fumigatus endophytic fungi suppress Pythium aphanidermatum and its induced damping-off diseases of cucumber and radish

Abstract

Damping-off diseases caused huge losses in crop production in numerous countries. Pythium aphanidermatum represents a major cause of these diseases. In the present investigation, two endophytic fungi Talaromyces omanensis and Aspergillus fumigatus isolated from a desert plant, Rhazya stricta, were investigated for their efficiency in suppressing P. aphanidermatum and its stimulated damping-off diseases in cucumber and radish. The results proved the ability of both endophytes to inhibit P. aphanidermatum growth in both solid and liquid forms, inhibit its oospore production, induce abnormal patterns in its hyphae and suppress its induced damping-off diseases. Moreover, T. omanensis produced an inhibition zone area against P. aphanidermatum and enhanced the fresh weight of cucumber and length, fresh weight and dry weight of radish. Further, A. fumigatus caused cellular leakage in P. aphanidermatum mycelium. The previous results were interpreted through the secretion of β-1,3-glucanase, cellulase and siderophore by both endophytic fungi.

Keywords:

• Biocontrol
• endophytic fungi
• damping-off
• β-1,3-glucanase
• cellulose
• siderophore

Acknowledgements

Thanks and gratitude to Prof. Sajeewa S.N. Maharachchikumbura for his help in phylogenetic analysis work and to Sultan Qaboos University for its financial support.

Disclosure statement

No potential conflicts of interest exist amongst the authors.