Estimation of β-cyfluthrin and imidacloprid in okra fruits and soil by chromatography techniques

Abstract

Dissipation of β-cyfluthrin and imidacloprid in okra was studied following three applications of a combination formulation of Solomon 300 OD (β-cyfluthrin 9 % + imidacloprid 21 %) @ 60 and 120 g a.i. ha⁻¹ at 7 days interval. Residues of β-cyfluthrin and imidacloprid in okra were estimated by gas liquid chromatography (GLC) and high performance liquid chromatography (HPLC), respectively. Residues of β-cyfluthrin were confirmed by gas chromatograph–mass spectrometry (GC-MS) and that of imidacloprid by high performance thin layer chromatography (HPTLC). Half-life periods for β-cyfluthrin were found to be 0.91 and 0.68 days whereas for imidacloprid these values were observed to be 0.85 and 0.96 days at single and double the application rates, respectively. Residues of β-cyfluthrin dissipated below its limit of quantification (LOQ) of 0.01 mg kg⁻¹ after 3 and 5 days at single and double the application dosage, respectively. Similarly, residues of imidacloprid took 5 and 7 days to reach LOQ of 0.01 mg kg⁻¹, at single and double dosages respectively. Soil samples collected after 15 days of the last application did not show the presence of β-cyfluthrin and imidacloprid at their detection limit of 0.01 mg kg⁻¹.

Keywords:

• β-cyfluthrin
• imidacloprid
• GLC
• HPLC
• GC-MS
• HPTLC
• half-life

Acknowledgment

The authors are thankful to Bayer CropScience, India and the Indian Council of Agricultural Research, India for sponsoring the project. Authors are also thankful to the Head, Department of Entomology, PAU, Ludhiana for providing research facilities.