Publication Cover
Journal of Environmental Science and Health, Part B
Pesticides, Food Contaminants, and Agricultural Wastes
Volume 48, 2013 - Issue 8
178
Views
4
CrossRef citations to date
0
Altmetric
Original Articles

The integration of in vivo and in vitro metabolism assays to investigate the stereoselective behaviors of benalaxyl in rainbow trout (Oncorhynchus mykiss)

&
Pages 693-699 | Received 22 Oct 2012, Published online: 02 May 2013
 

Abstract

The bioconcentration and elimination of racemic benalaxyl (BX) in trout liver microsomes and in juvenile rainbow trout (Oncorhynchus mykiss) were investigated to determine whether the fish can bioconcentrate and degrade this fungicide enantioselectively. Both enantiomers of BX were extracted with organic solvents and evaluated using high-performance liquid chromatography. In the microsomes, BX degradation followed first-order kinetics, and the S−(+) enantiomer of BX was eliminated twice as rapidly as the R−(−) enantiomer, resulting in residues enriched for R−(−)−BX. In vivo experiment, chiral analysis showed an obvious selective bioconcentration of BX based on statistically altered enantiomer fractions (EFs) in the fish compared with the values in the water. The R−(−)−BX was initially preferentially bioconcentrated by rainbow trout and then dissipated more slowly than its antipode. The mean half-lives for individual enantiomers were calculated as 31.6 h for R−(−)−BX and 20.3 h for the S−(+)form. The results of the study showed that the degradation of BX enantiomers was stereoselective in rainbow trout.

View correction statement:
Corrigendum

Acknowledgment

The authors gratefully acknowledge the support from the key laboratory of feed biotechnology, ministry of agriculture of China and the support form of Feed Research Institute, Chinese Academy of Agricultural Sciences.

Log in via your institution

Log in to Taylor & Francis Online

PDF download + Online access

  • 48 hours access to article PDF & online version
  • Article PDF can be downloaded
  • Article PDF can be printed
USD 61.00 Add to cart

Issue Purchase

  • 30 days online access to complete issue
  • Article PDFs can be downloaded
  • Article PDFs can be printed
USD 711.00 Add to cart

* Local tax will be added as applicable

Related Research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.