Influence of the pesticides glyphosate, chlorpyrifos and atrazine on growth parameters of nonochratoxigenic Aspergillus section Nigri strains isolated from agricultural soils

Abstract

This investigation was undertake to determine the effect of glyphosate, chlorpyrifos and atrazine on the lag phase and growth rate of nonochratoxigenic A. niger aggregate strains growing on soil extract medium at −0.70, −2.78 and −7.06 MPa. Under certain conditions, the glyphosate concentrations used significantly increased micelial growth as compared to control. An increase of about 30% was observed for strain AN 251 using 5 and 20 mg L⁻¹ of glyphosate at −2.78 MPa. The strains behaved differently in the presence of the insecticide chlorpyrifos. A significant decrease in growth rate, compared to control, was observed for all strains except AN 251 at −2.78 MPa with 5 mg L⁻¹. This strain showed a significant increase in growth rate. With regard to atrazine, significant differences were observed only under some conditions compared to control. An increase in growth rate was observed for strain AN 251 at −2.78 MPa with 5 and 10 mg L⁻¹ of atrazine. By comparison, a reduction of 25% in growth rate was observed at −7.06 MPa and higher atrazine concentrations. This study shows that glyphosate, chlorpyrifos and atrazine affect the growth parameters of nonochratoxigenic A. niger aggregate strains under in vitro conditions.

Keywords:

• Glyphosate
• chlorpyrifos
• atrazine
• Aspergillus niger aggregate
• soil-based medium

Acknowledgments

C.S. Carranza is a fellow from Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), C.L. Barberis, S.M. Chiacchiera and C.E. Magnoli are members of the research career of CONICET.

Funding

This work was supported by Agencia Nacional de Promoción Científica y Tecnológica (ANPCYT-PICT- 2482/10) and Secretaría de Ciencia y Técnica, Universidad Nacional de Río Cuarto (SECYT-UNRC- 18/C391).