Zweigelt and Niagara skin extracts suppress cyclobutane pyrimidine dimer formation due to UV irradiation in NHEK cells: first attempt

Abstract

The grape skins after pressing the juice are a major problem for winery. However, because it contains a large amount of polyphenols, development of effective usages are expected to construct sustainable waste use. In this study, we examined whether grape skin extract is effective for recovery of DNA damage caused by UV irradiation. Extract from Zweigelt and Niagara skin was prepared by methanol, and UV irradiation was performed at 10 mJ/cm² (250 nm) and 15 mJ/cm² (290 nm) using human normal skin cells. As results, the decreased cell viability due to UV irradiation was improved by adding Niagara or Zweigelt skin extract. On the other hand, cyclobutane pyrimidine dimer production due to UV irradiation decreased significantly by Niagara or Zweigelt extract. In addition, the effects of grape skin extracts on the expression of sirtuin gene were also examined.

Keywords:

• Sirtuin
• ultraviolet irradiation
• Zweigelt
• Niagara
• polyphenols

Acknowledgments

The authors thank to Ms. Miyako Komori for her technical help about cell experiments.

Correction Statement

This article has been republished with minor changes. These changes do not impact the academic content of the article.

Additional information

Funding

This work was supported by the Joint research fund with Hokkaido Wine Co., Ltd with Hokkaido University; however, the authors declare that there is no conflict of interest.