Fabrication of Interpenetrating Networks of Poly (vinyl alcohol-g-acrylamide) and Chitosan-g-polyacrylamide Chains and Evaluation of Water Sorption, Blood Compatibility and Cytotoxicity Behaviors

Abstract

In the present study an interpenetrating polymer network (IPN) of poly (vinyl alcohol-g-acrylamide) and chitosan-g-polyacrylamide chains were prepared by the redox polymerization method. The prepared network was characterized by FTIR, DSC and ESEM techniques. The hydrogels were evaluated for their water intake potential and influence of various factors such as chemical composition of the hydrogels, pH and temperature of the swelling bath; various simulated biological fluids were investigated on the water sorption capacity of the IPNs. The in-vitro blood compatibility of the prepared IPN was judged by blood-clot formation, percent haemolysis, protein (BSA) adsorption, and cytotoxicity methods.

Keywords:

• Biocompatibility
• Cytotoxicity
• Hydrogel
• Redox polymerization
• Swelling

ACKNOWLEDGMENTS

The authors would like to acknowledge IUC-DAE Consortium for Scientific Research Indore (M.P.), India for providing SEM, DSC, and FTIR facilities and Shree Chitra Tirunal Institute of Biomedical Science, Thiruannathpurum, India for conducting the cytotoxicity tests.