Enhanced Production of Polyhydroxybutyrate Using Enterococcus faecium KT722772 Isolated from Cattle Rumen Fluid by Substrates Optimization

ABSTRACT

Poly(3-hydroxybutyrate)-producing Enterococcus faecium KT722772 was isolated from cattle rumen fluid by Sudan black staining. It was found to be gram-positive cocci. Poly(3-hydroxybutyrate) production using E. faecium KT722772 was optimized at different pH ranging from 2 to 7 and with different substrates such as glucose, butyric acid, propionic acid, sodium acetate, and glycerol as a sole carbon source at a concentration ranging from 0 to 4%. Maximum poly 3-hydroxy butyrate production (1.8 g/L) was achieved with 1% butyric acid at pH 7. Poly 3-hydroxy butyrate produced by E. faecium was characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction analysis.

GRAPHICAL ABSTRACT

KEYWORDS:

• Butyric acid
• polyhydroxybutyrate
• rumen fluid