High-Performance Liquid Chromatography of Hop Compounds in Brewing¹

1 Presented at the 41st Annual Meeting, Kiamesha Lake, N.Y., May 1975.

Abstract

The separation of hop-derived compounds with several high-performance liquid chromatography techniques was studied. A system employing ion-exchange materials was developed which clearly resolves β-, α-, and iso-α-acids in less than 2 hr without gradient elution. Adsorption and partition columns can also separate the major groups of hop compounds. Reverse-phase partition chromatography is capable of resolving the cis- and trans-iso-α-acids.

• Adsorption
• α- and Iso-α-acids
• β-Acids
• Ion exchange
• Partition