First Report of Hb Limassol [β8(A5)Lys→Asn; HBB: c.27G>C] from Odisha, India

Abstract

In this short report, we describe the clinical presentation of a rare hemoglobin (Hb) variant, Hb Limassol [β8(A5)Lys→Asn; HBB: c.27G>C] with a faster electrophoretic mobility than Hb A and that elutes in the P3 window on cation exchange high performance liquid chromatography (HPLC). This sequence variation at codon 8 (AAG>AAC) of the HBB gene was found in the four heterozygous cases, all of whom were clinically asymptomatic.

Keywords:

• Alkaline electrophoresis
• cation exchange high performance liquid chromatography
• DNA sequencing
• Hb Limassol

Acknowledgements

Thanks are due to Professor Dr Sunamali Bag, Director, VIMSAR, Burla, Sambalpur, Odisha, India, and Professor Bijaya Kumar Dutta, Dean and Principal, VIMSAR, Burla, Sambalpur, Odisha, India. The authors are indebted to the late Dr Dilip Kumar Patel, Associate Professor, Department of Medicine, Veer Surendra Sai Medical College, Burla, Sambalpur, Odisha and former Project Coordinator, Odisha Sickle Cell Project, NHM, Odisha, India, for his inestimable contribution to this study.

Disclosure statement

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article.

Additional information

Funding

The study was performed under the Odisha Sickle Cell Project, funded by the National Health Mission Odisha, Department of Health & Family Welfare, Government of Odisha, India. The authors acknowledge the support of the Director, Anthropological Survey of India, Ministry of Culture, Government of India, for sanctioning the collaboration program (Vide letter No. 18-22/PMI/2011, dated June 15 2013).